![]() (By setting this popup menu to "Off", you are telling FlowJo to use all of the original graphs that dragged and dropped into the layout). By selecting any of these values from this menu, FlowJo will change the current layout view to show graphs & statistics drawn only from tubes with that value of the Iterator. These are then displayed in the "Iteration" popup menu. Then you can place multiple graphs from different tubes (from different FCS files!) into the same layout and still generate a batch output.įor example, if you set the iteration attribute to be the keyword "$Cells", where you have a Patient ID entered into the $Cells keyword, then FlowJo will examine all of the tubes in the current workspace, and generate a list of the unique values of $Cells. drop-down box you can select a keyword that groups tubes into distinct units-for example, a keyword that connotes patient ID, tissue type, or experimental condition. This is described in more detail at the bottom of this page.įrom the Iterate over. This field is the mechanism for finding the correct sample, when more than one sample is collected with the same iteration value. In advanced reporting, there is an additional feature known as the discriminator. When we talk about iteration attribute we mean the attribute that is changed for each frame of the stack that is generated, for instance the date of the collection. For example, most files will contain, in their header, a $Date attribute, and a value containing the date on which that sample was collected. The value is the data recorded for a particular tube. The attribute is the name for the type of data recorded. ![]() The keywords in an FCS file are organized in attribute-value pairs. There are a couple of terms that we use in this explanation that are important to get clear from the start. to set more parameters for your batch operation: More sophisticated organization is required. In cases such as these, it is not enough to simply process successive tubes in a batch report. Examples of multi-sample reports could be several tissues from a common animal, multiple doses of a common drug, or cross patient studies using blood collected over time. Other times, reports are created where each frame contains data from more than one tube. This is useful in cases where the same information is desired for each tube. The batch report is made by taking each sample, applying the named gates and statistics to that sample, plotting the graphics in the format defined by the layout editor, and adding that frame to the report. In the simple case, each frame corresponds to one sample in a group. In every case, FlowJo will produce a series of frames, each one containing graphs and statistics from one or more tubes. Alternatively, it can print the report directly to your printer.A web page containing the pictures for each layout group, or.A preview window containing static tiles (data won't update if you change the original analysis.).The result of a batch report can be viewed in several different ways.
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